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Structure of various DI RNAs. 25CAT RNA (25) contains a wild-type leader RNA sequence; 25-Δ4R-CAT contains a mutant leader RNA without the four UCUAA repeats. IG 7 is the promoter sequence for gene 7; 25CAT/RIS is similar to 25 CAT but lacks the IG site (25). The plasmids for these RNAs were made by PCR amplification of p25CAT with appropriate primers.
