Fig. 2.

The structure of targeting constructs (A) and the efficiency of their mitochondrial targeting (B). (A) CMV, the human cytomegalovirus promoter; MTS, one of the six mitochondrial targeting sequences used; FP, either DRE or mCherry; pA, polyadenylation site. (B) The red fluorescence (panels labeled FP) indicates the distribution of DRE or mCherry, the green fluorescence (panels labeled MT) shows mitochondrial distribution as revealed by MitoTracker Green Staining, and the panels labeled OL represent an overlay of two images. The areas of yellow color indicate mitochondrial localization of FP