Table 1.
Primers used in this study
| Name | Sequence* |
|---|---|
| mCherryPCRf | GCGGGATCCGTGAGCAAGGG |
| mCherry PCRr | CGCCTCGAGTCACTTGTACAGC |
| DsExprFnoATGbam | GCGGGATCCGCCTCCTCCGAGGACGTCAT |
| DsExprRxba | CGCTCTAGATTACAGGAACAGGTGGTGGCGGC |
| mCherrySHORTf | GCGGGATCCGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGGTTC |
| mCherrySHORTr | CGCCTCGAGTTACAGGAACAGGTGGCTGTGCCGGCCCTCGGCCCG |
| MD1/DM1F | GAAGCACCCCGCCGACATCCCC |
| MD1/DM1R | GGGGATGTCGGCGGGGTGCTTC |
| MD2F | CGACGGCCCCGTAATGCAGAAGAAGAC |
| MD2R | GTCTTCTTCTGCATTACGGGGCCGTCG |
| MD3F | CTGAAGCTGAAGGACGGCGGCCACTAC |
| MD3R | GTAGTGGCCGCCGTCCTTCAGCTTCAG |
| MD4F | GCCAAGAAGCCCGTGCAGCT |
| MD4R | AGCTGCACGGGCTTCTTGGC |
| DsRes M182Kf | CAAGTCCATCTACAAGGCCAAGAAGCCC |
| DsRes M182Kr | GGGCTTCTTGGCCTTGTAGATGGACTTG |
| mCherry1 | GCGGGATCCGTGAGCAAGGGCGAGGAGGACAACATGGCCATCATCAAGGAGTTCATG |
| mCherry2 | CGTGGCCGTTCACGCTGCCCTCCATGTGCACCTTGAACCGCATGAACTCCTTGATGATGG |
| mCherry3 | GGGCAGCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGGCCCTACGA |
| mCherry4 | GCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGCCGGCCCTCGCC |
| mCherry5 | TGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGAGCCCCCAGTTCA |
| mCherry6 | ATGTCGGCGGGGTGCTTCACGTAGGCCTTGCTGCCGTACATGAACTGGGGGCTCAGGATG |
| mCherry7 | GTGAAGCACCCCGCCGACATCCCCGACTACCTGAAGCTGAGCTTCCCCGAGGGCTTCAAG |
| mCherry8 | TCACCACGCCGCCGTCCTCGAAGTTCATCACCCGCTCCCACTTGAAGCCCTCGGGGAAGC |
| mCherry9 | CGAGGACGGCGGCGTGGTGACCGTGACCCAGGACAGCAGCCTCCAGGACGGCGAGTTCAT |
| mCherry10 | GTCGCTGGGGAAGTTGGTGCCCCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGGAG |
| mCherry11 | GCACCAACTTCCCCAGCGACGGCCCCGTGATGCAGAAGAAGACAATGGGCTGGGAGGCCA |
| mCherry12 | CCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGCTGCTGGCCTCCCAGCCCATTGTC |
| mCherry13 | GAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCAC |
| mCherry14 | GCTTCTTGGCCTTGTAGGTGGTCTTCACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCT |
| mCherry15 | CACCTACAAGGCCAAGAAGCCCGTGCAGCTCCCCGGCGCCTACAACGTGAACATCAAGCT |
| mCherry16 | CTCCACGATGGTGTAGTCCTCGTTGTGGCTGGTGATGTCCAGCTTGATGTTCACGTTGTA |
| mCherry17 | AGGACTACACCATCGTGGAGCAGTACGAGCGGGCCGAGGGCCGGCACAGCACCGGCGGCA |
| mCherry18 | CGCCTCGAGTCACTTGTACAGCTCGTCCATGCCGCCGGTGCTGTGCCGG |
*
Restriction sites are in boldface, italicized, and underlined