Table 2.
Subcellular partitioning of fusion constructs
| MTS | mCherry |
mCherry short |
Dsred Express |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Mito | Mix | Cyto | Mito | Mix | Cyto | Mito | Mix | Cyto | |
| Cox | 0 | 98.7 ± 1.3 | 1.3 ± 1.3 | 12.7 ± 4.3 | 87.3 ± 4.3 | 0 | 92 ± 1 | 8 ±1 | 0 |
| OTC | 48.3 ± 3.3 | 44.3 ± 2.6 | 7.3 ± 2.9 | 83.7 ± 7.8 | 16.3 ± 8 | 0 | 99 ± 1 | 1 ± 1 | 0 |
| TFAM | 7.3 ± 1.9 | 92.7 ± 1.9 | 0 | 4 ± 2 | 96 ± 2 | 0 | 100 | 0 | 0 |
| MDH2 | 0 | 34.3 ± 11.9 | 65.7 ± 11.9 | 0.7 ± 0.7 | 98.3 ± 0.9 | 0 | 75 ± 4.7 | 24.5 ± 4.7 | 0.25 ± 0.25 |
| hPolG | 0 | 1.3 ± 1.3 | 98.7 ± 1.3 | 0 | 2.3 ± 1.2 | 97.7 ± 1.2 | 68.3 ± 5.4 | 31.7 ± 5.4 | 0 |
| hSOD | 0 | 7.7 ± 5 | 92.3 ± 5 | 0 | 5 ± 2.5 | 95 ± 2.5 | 67.7 ± 2.3 | 32.3 ± 2.3 | 0 |
The HeLa cells were transfected and the percentage of cells showing mitochondrial, cytoplasmic, or mixed localization of expressed FP was scored as described in Materials and Methods. The data are mean ± SEM of at least three independent transfections