TABLE 3.
Antigenicity of MV F protein mutated at amino acid 73a
| F protein | Anti-FMab
|
|||
|---|---|---|---|---|
| Ost 3 | F 186 | F 263 | Fusion | |
| Hallé | + | + | + | + |
| Hallé-mar | + | − | + | + |
| Lys-1 | + | − | + | + |
| Hallé R73K | + | − | + | + |
| Lys K73R | + | + | + | + |
a
HeLa cells were infected with 0.1 PFU/cell of a vaccinia recombinant virus coding for the MV H (4) and then transfected by the Lipofectin method (Gibco-BRL) with plasmids coding for the F protein under the control of the vaccinia H6 promoter (10). The cells were observed for fusion and antigen expression by immunofluorescence 20 h later. Site-directed mutagenesis was carried out with M13 phage as previously described (20).