Figure 3. Western blot quantification of mutant Me31B protein levels in me31B^E208A, me31B^DVLAAAA, and me31B^R385Q heterozygous strains.

(A) Anti-GFP Western blots were used to quantify the Me31B^WT-GFP, Me31B^E208A-GFP, Me31B^DVLAAAA-GFP, and Me31B^R385Q-GFP proteins in the ovaries of the corresponding heterozygous strains. Anti-tubulin Western blots were used as loading controls. The expression levels of Me31B^E208A-GFP, Me31B^DVLAAAA-GFP, and Me31B^R385Q-GFP proteins were lower than the Me31B^WT control. The images shown are representative images of three biological replicates. The additional, uncropped biological replicate images are presented in Supplementary Figure 1. (B) The Me31B^E208A-GFP, Me31B^DVLAAAA-GFP, and Me31B^R385Q-GFP protein levels are 68% (p > 0.05), 53% (p < 0.05), and 23% (p < 0.05) relative to the Me31B^WT-GFP control, respectively. Western blot image analysis was performed with ImageJ and protein quantification was normalized by using the alpha-tubulin proteins. NS, not statistically significant. Error bar represents the standard error of the mean.