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. 1999 Jan;73(1):834–838. doi: 10.1128/jvi.73.1.834-838.1999

FIG. 2.

FIG. 2

Western blots of cell lysates of CRFK cells transfected with various constructs and stained with FCV-specific Mab K. (A) Comparison of the expression efficiencies of the CMV, SV40, and T7 promoter constructs. Lane 1, pCAPI (CMV promoter) lysate of about 5 × 106 cells; lane 2, pCAPIV (SV40 promoter) lysate of about 5 × 106 cells; lane 3, pCAPI plus MVA/T7 (T7 promoter) lysate of about 5 × 105 cells; lane 4, MVA/T7-infected control cells (5 × 106 cells); lane 5, CsCl gradient-purified virus FCV-KS20. (B) Processing of the capsid protein precursor and the mature capsid protein in CRFK cells. Lane 1, precursor capsid protein gene expressed with MVA/T7. Note the processing of the capsid protein precursor to the 62-kDa mature-size protein; lane 2, expression of the mature truncated capsid protein gene expressed with MVA/T7. (C) Processing of the capsid protein precursor in nonpermissive cell lines: murine L929 cells (lanes 1 to 4) and Vero cells (lanes 5 to 8). Lanes 1 and 5, precursor gene expressed with MVA/T7; Lanes 2 and 6, mature truncated capsid protein gene expressed without MVA/T7; lanes 3 and 7, mature truncated capsid protein expressed with MVA/T7, lanes 4 and 8, MVA/T7-infected control cells.