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. 2020 Sep 15;133(18):jcs247593. doi: 10.1242/jcs.247593

Fig. 2.

Fig. 2. DRP1 is essential for the thermogenic program of beige and brown adipocytes. (A) Immunoblots and (B) qPCR analysis of thermogenic genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (C) Immunoblots and (D) qPCR analysis for mitochondrial genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (E) Immunoblots and (F) qPCR analysis of mitochondrial dynamics-related genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (G) Immunoblots of mitochondrial dynamics- and adipogenesis-related proteins in the SVF cells of brown adipose tissue differentiated into brown adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (H) Immunoblots for DRP1, thermogenic-, mitochondrial content- and mitochondrial dynamics-related proteins in SVF cells transfected with scrambled siRNA (siScr) or siRNA against Drp1 (siDRP1) and then differentiated into beige adipocytes. Data for the differentiation assays was collected after 6 days of differentiation. Bar graphs are presented as mean±s.e.m. (**P<0.01 ***P<0.001, ****P<0.0001). Und, undifferentiated. Actin is shown as loading control for immunoblots, and β-actin was used to normalize gene expression.

DRP1 is essential for the thermogenic program of beige and brown adipocytes. (A) Immunoblots and (B) qPCR analysis of thermogenic genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (C) Immunoblots and (D) qPCR analysis for mitochondrial genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (E) Immunoblots and (F) qPCR analysis of mitochondrial dynamics-related genes in SVF cells differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (G) Immunoblots of mitochondrial dynamics- and adipogenesis-related proteins in the SVF cells of brown adipose tissue differentiated into brown adipocytes in the presence or absence of 10 µM mdivi-1 added from day 0 of differentiation. (H) Immunoblots for DRP1, thermogenic-, mitochondrial content- and mitochondrial dynamics-related proteins in SVF cells transfected with scrambled siRNA (siScr) or siRNA against Drp1 (siDRP1) and then differentiated into beige adipocytes. Data for the differentiation assays was collected after 6 days of differentiation. Bar graphs are presented as mean±s.e.m. (**P<0.01 ***P<0.001, ****P<0.0001). Und, undifferentiated. Actin is shown as loading control for immunoblots, and β-actin was used to normalize gene expression.