Skip to main content
. 2020 Sep 15;133(18):jcs247593. doi: 10.1242/jcs.247593

Fig. 5.

Fig. 5. DRP1 is dispensable during the late phase of beige adipocyte differentiation. SVF cells were differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added either from day 0 (D0) or day 2 (D2) of differentiation and continued until 6 days of differentiation. (A) Representative images of the differentiated beige adipocytes. Scale bars: 500 µm. (B) Immunoblots and (C) qPCR analysis of adipogenesis-related genes. (D) Immunoblots and (E) qPCR analysis of mitochondria-related genes in the differentiated beige adipocytes. Data for the differentiation assays was collected after 6 days of differentiation. β-actin was used to normalize gene expression and actin immunoblots are shown as a loading control. Bar graphs are presented as mean±s.e.m. (***P<0.001). Und, undifferentiated.

DRP1 is dispensable during the late phase of beige adipocyte differentiation. SVF cells were differentiated into beige adipocytes in the presence or absence of 10 µM mdivi-1 added either from day 0 (D0) or day 2 (D2) of differentiation and continued until 6 days of differentiation. (A) Representative images of the differentiated beige adipocytes. Scale bars: 500 µm. (B) Immunoblots and (C) qPCR analysis of adipogenesis-related genes. (D) Immunoblots and (E) qPCR analysis of mitochondria-related genes in the differentiated beige adipocytes. Data for the differentiation assays was collected after 6 days of differentiation. β-actin was used to normalize gene expression and actin immunoblots are shown as a loading control. Bar graphs are presented as mean±s.e.m. (***P<0.001). Und, undifferentiated.