Extended Data Table 1.
Metabolic concentrations (μM) detected using NMR
| Compound | Pre-drought | Drought | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | Formula | P15-0hr | P24-0hr | P26-0hr | P34-0hr | P35-0hr | P12-Ctrl | P15-0hr | P24-6hr | P26-0hr | P34-0hr | P34-0hr |
| 2-Oxoisocaproate**P | C6H10O3 | 6 | 5 | D | 5 | D | ND | ND | ND | ND | ND | D |
| 3-Hydroxybutyrate | C4H8O3 | ND | ND | ND | ND | D | ND | ND | ND | ND | ND | ND |
| Acetate | C2H4O2 | 22 | 38 | 30 | 55 | 26 | 19 | 35 | 31 | 31 | 24 | 11 |
| Acetone | C3H6O | ND | D | D | ND | ND | D | ND | ND | ND | ND | D |
| Alanine**P | C3H7NO2 | 24 | 21 | 15 | 20 | 17 | 18 | 9 | 13 | 9 | 7 | 9 |
| Benzoate | C7H6O2 | ND | ND | ND | D | ND | ND | ND | ND | ND | ND | ND |
| Betaine | C5H11NO2 | ND | ND | ND | ND | ND | D | D | ND | ND | ND | ND |
| Ethanol | C2H6O | 12 | 17 | 21 | 19 | 18 | 28 | 24 | 12 | 22 | 22 | 12 |
| Formate***P | CH2O2 | 17 | 38 | 40 | 23 | 31 | ND | D | 6 | 16 | D | 11 |
| Glutamate | C5H9NO4 | 12 | 12 | 8 | 6 | 10 | 11 | 7 | 10 | 10 | 10 | 6 |
| Glycerol | C3H8O3 | 6 | 8 | 8 | 6 | 7 | 5 | 6 | 7 | 5 | 8 | 6 |
| Glycine*P | C2H5NO2 | 12 | 9 | 6 | 8 | 7 | 5 | D | 7 | 6 | D | D |
| Isoleucine | C6H13NO2 | 7 | 7 | D | D | D | D | D | D | D | D | D |
| Lactate | C3H6O3 | 6 | 7 | D | 12 | 7 | D | D | 11 | D | D | 7 |
| Leucine*P | C6H13NO2 | 12 | 11 | 7 | 7 | 6 | 7 | 6 | 6 | 6 | D | D |
| Methanol | CH4O | 150 | 375 | 367 | 222 | 326 | 508 | 329 | 203 | 370 | 372 | 322 |
| Phenylalanine*P | C9H11NO2 | D | D | ND | D | ND | ND | ND | ND | ND | ND | ND |
| Pyroglutamate*P | C5H7NO3 | 22 | 19 | 10 | 16 | 9 | 8 | 5 | 12 | 8 | D | D |
| Pyruvate**P | C3H4O3 | 11 | 13 | 10 | 11 | 10 | D | 5 | 9 | 5 | D | 7 |
| Succinate | C4H6O4 | ND | ND | ND | ND | ND | ND | D | D | ND | ND | ND |
| Threonine | C4H9NO3 | 6 | 7 | D | D | D | D | D | D | D | D | D |
| Trehalose*D | C12H22O11 | ND | ND | ND | ND | ND | 18 | D | 7 | 6 | 13 | D |
| Uracil*P | C4H4N2O2 | D | 5 | D | D | D | ND | ND | D | ND | ND | D |
| Valine*P | C5H11NO2 | 15 | 13 | 8 | 9 | 8 | 8 | 6 | 8 | 6 | 5 | 5 |
Samples displayed are a subset of total samples based on the quality of NMR data. Most samples are from 0 h, except for P24 during drought which is from 6 h post pyruvate injection.
ND, not detected (below the level of detection where concentration < 1.9 μM); D, detected (below level of quantification, where concentration 2 - 5 μM)
P, Compound higher during pre-drought (Linear Fixed Effect Model (d.f. = 7), where ND and D were given values of 1 and 4 μM respectively, Oxoisocaproate P=0.0014, Alanine P=0.0074, Formate P=4.2E-4, Glycine P=0.017, Leucine P=0.016, Phenylalanine P=0.030, Pyroglutamate P=0.018, Pyruvate P=0.0011, Uracil P=0.022, Valine P=0.012)
D, Compound higher during drought (Trehalose P=0.020)
*, P<0.05; **, P<0.01; ***, P<0.001