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. 2023 Jul 31;14:4494. doi: 10.1038/s41467-023-40201-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The Pla2g6^+/+ and Pla2g6^–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Survival ratio after TAC. n = 17 (sham-operated Pla2g6^+/+), 13 (sham-operated Pla2g6^–/–), 51 (TAC-operated Pla2g6^+/+), and 51 (TAC-operated Pla2g6^–/–). The log-rank test was used for survival analysis. Sham Pla2g6^+/+ versus TAC Pla2g6^+/+ P = 0.0000001, TAC Pla2g6^+/+ versus TAC Pla2g6^–/– P = 0.0002. b Representative images of transthoracic M-mode echocardiographic tracing (scale bars, 0.2 s and 5 mm, respectively) and the echocardiographic parameters of the mice. n = 7 (sham-operated Pla2g6^+/+), 7 (sham-operated Pla2g6^–/–), 14 (TAC-operated Pla2g6^+/+) and 10 (TAC-operated Pla2g6^–/–). LVIDd and LVIDs, end-diastolic and end-systolic left ventricular (LV) internal dimensions; FS, fractional shortening of LV. c Physiological parameters of the mice. n = 7 (sham-operated Pla2g6^+/+), 7 (sham-operated Pla2g6^–/–), 14 (TAC-operated Pla2g6^+/+), and 10 (TAC-operated Pla2g6^–/–), biologically independent samples. d Representative images of the hematoxylin—eosin-stained heart sections. Experiment was repeated five times independently with similar results. Scale bar, 100 μm. e Representative images of the wheat germ agglutinin (green)-stained heart sections. Scale bar, 50 μm. Blue, DAPI. The graph shows the cross-sectional area of cardiomyocytes (n = 5, biologically independent samples). f Representative images of Azan—Mallory-stained heart sections. Scale bar, 100 μm. The graph shows the ratio of the fibrotic area to whole heart section (n = 5, biologically independent samples. Sham Pla2g6^+/+ versus TAC Pla2g6^+/+ P = 0.0000000002, sham Pla2g6^–/– versus TAC Pla2g6^–/– P = 0.000000001). g Expression levels of mRNAs related to cardiac remodeling. (n = 6, biologically independent samples. Sham Pla2g6^+/+ versus TAC Pla2g6^+/+ in Nppb/Gapdh P = 0.00006). Data were normalized to the Gapdh content and are expressed as the fold increase over the levels in the sham-operated Pla2g6^+/+ group. Open and closed bars indicate Pla2g6^+/+ and Pla2g6^−/−, respectively. In violin plots, solid lines show median. In bar graphs, data are expressed as the mean ± SEM. The data were evaluated by one-way ANOVA, followed by Tukey-Kramer’s post-hoc test. Source data are provided as a Source Data file.