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. 2023 Jul 31;14:4494. doi: 10.1038/s41467-023-40201-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The Pla2g6^+/+ and Pla2g6^–/– mice were subjected to transverse aortic constriction (TAC) and then analyzed 5 days after the operation. a Representative confocal images of TUNEL-positive cardiomyocytes. Arrow heads indicate TUNEL (green)-positive nuclei. Scale bar, 20 μm. Red, α-sarcomeric actin; blue, DAPI. The graph shows quantitative analysis of TUNEL-positive cardiomyocytes (n = 5, biologically independent samples). b Serum troponin T levels. n = 5 (sham-operated Pla2g6^+/+), 5 (sham-operated Pla2g6^–/–), 8 (TAC-operated Pla2g6^+/+), and 8 (TAC-operated Pla2g6^–/–), biologically independent samples. c Uptake of Evans blue dye (red) in the hearts of Pla2g6^+/+ and Pla2g6^–/– mice following TAC or sham operation. Scale bar, 1 mm. The graph shows the ratio of Evans blue dye-positive area to left ventricular area. n = 5 (sham-operated Pla2g6^+/+), 5 (sham-operated Pla2g6^–/–), 7 (TAC-operated Pla2g6^+/+), and 7 (TAC-operated Pla2g6^–/–) biologically independent samples. d Immunofluorescence analysis of HMGB1 (green) and α-sarcomeric actin (red) in the heart after TAC (n = 5, biologically independent samples. Sham Pla2g6^+/+ versus TAC Pla2g6^+/+ P = 0.00002). Arrow heads indicate HMGB1-negative nuclei. Scale bar, 50 μm. Blue, DAPI. The graph shows the percentage of HMGB1-negative cardiomyocytes. Open and closed bars indicate Pla2g6^+/+ and Pla2g6^−/−, respectively. Data are expressed as the mean ± SEM. The data were evaluated by one-way ANOVA, followed by Tukey-Kramer’s post-hoc test. Source data are provided as a Source Data file.