Skip to main content
. 2023 Jul 31;14:4600. doi: 10.1038/s41467-023-40314-w

Fig. 4. Schwann cells promote malignant progression in PDAC via MDK.

Fig. 4

a–d Effects of RSC96 CM (a, c) and sNF96.2 CM (b, d) on migration (a, b) and invasion (c, d) of Panc-1 and CFPAC-1 cells were assessed by Transwell and Matrigel invasion assays. n = 10, 15, 16 (a) or 5 (b–d) representative pictures over three independent experiments. Scale bar, 200 µm. e, f The relative expression levels of EMT markers in Panc-1 and CFPAC-1 cells cultured with RSC96 CM were detected by qPCR (e) and western blotting (f). Data were representative of n = 3 independent experiments. g–j Effects of RSC96 CM and sNF96.2 CM on the cell proliferation of Panc-1 and CFPAC-1 cells were assessed by CCK-8 (g, h), flow cytometry (i), and EdU assays (j). Scale bar, 100 µm. k QuSAGE scores of control and SC-CM associated signature in perineural tier 1‒4 and other regions in the neuro-stroma niche. The signatures were based on the bulk RNA-seq of CFPAC-1. l, m Effects of recombinant human MDK on migration (l) and proliferation (m) of Panc-1 cells were assessed by Transwell (l) and CCK-8 assay (m). Scale bar, 200 µm. n, o. Effects of MDK neutralization antibodies on migration (n) and proliferation (o) of Panc-1 cells cultured with RSC96 CM were assessed by Transwell (n) and CCK-8 (o) assays. Scale bar, 200 µm. e, g–j, m, n Data were the mean ± s.d. of n = 3 independent experiments. l, o Data were the mean ± s.d. of n = 5 independent experiments. Statistical analysis: unpaired two-sided t-test (a–e, i, j, l, n); two-way ANOVA (g, h, m, o). Source data are provided as a Source Data file.