FIG. 2.

(A) Schematic diagram of the US11 promoter. Putative promoter elements located within the US11 promoter (Fig. 1) and nested deletions generated within the US11 promoter are indicated. (B) Activation of the US11 promoter by the IE proteins. The US11 promoter and the indicated deletion mutants were cotransfected into HFFs with a construct (pSVH) expressing the IE gene region under the control of the MIEP. Cells were harvested 48 h after transfection and assessed for CAT activity. Activity is expressed relative to the wild-type promoter at 100%. Except for the d40 deletion, the average (± standard deviation) of at least three experiments is represented. Results for the d40 deletion represent data from two experiments. ∗, P < 0.001 (student’s t test).