TABLE 2.
Mutant virus cpts530/1030 is highly attenuated in the upper and lower respiratory tracts of chimpanzees
| Inoculuma | No. of chimpanzees | Reference | Mean peak virus titer (log10 PFU/ml) in:
|
Rhinorrhea score
|
No. of days with cough | ||
|---|---|---|---|---|---|---|---|
| Nasal wash | Tracheal lavage | Peak | Meanb | ||||
| A2 (wt) | 2 | 7, 8 | 5.0 ± 0.35 | 5.5 ± 0.40 | 3.0 | 1.6 | 1.0 |
| cpRSV | 2 | 7, 8 | 4.7 ± 0.40 | 2.9 ± 0.10 | 1.0 | 0.6 | 0.0 |
| cpts530 | 4 | 9 | 4.1 ± 0.44 | 1.1 ± 0.50 | 2.0 | 0.5 | 1.0 |
| cpts530/1030 | 2 | This study | 2.5 ± 0.01 | 0.8 ± 0.14 | 0.0 | 0.0 | 0.0 |
a
Chimpanzees were inoculated simultaneously by the intranasal and intratracheal routes with 104 PFU of the indicated virus at each site. Nasal wash samples were collected daily for 10 days, and tracheal lavage samples were collected on days 2, 5, 6, 8, and 10.
b
Mean of the scores obtained during the 8 days of peak virus shedding.