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. 2023 May 16;73(1):52–60. doi: 10.3164/jcbn.22-140

Fig. 3.

Fig. 3.

Identification of unknown CoQ10 reducing activity in rat liver homogenate. (A) HPLC chromatogram of a concentrated fraction containing the CoQ10 reducing compound. The reducing compound was detected at 6.4 min of retention time. (B) MS spectra of the CoQ10 reducing compound (upper) and RF standard (lower) as measured by optimized TOFMS with positive ESI. (C) HPLC chromatograms of a reaction mixture containing RF (10 μM), NADH (2.0 mM), and CoQ10 (70 μM) after 0 min (upper) and 60 min (lower) incubation at 37°C. (D) Chemical structures of RF, FMN, and FAD. (E) Time course of CoQ reduction induced by NADH (2.0 mM) and 10 μM of RF (○), FMN (□), and FAD (△) during incubation at 37°C. (F) Dependency of CoQ10 reduction on concentrations of RF compounds. Initial reduction rate was measured during CoQ10 (100 μM) reduction with RF (○), FMN (△), or FAD (□) in the presence of NADH (2.0 mM), and with RF (×), FMN (▲), or FAD (◇) in the absence of NADH.