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. 2023 Jul 18;14:1224958. doi: 10.3389/fpls.2023.1224958

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Copyright © 2023 Ershova, Kamarova, Sheshukova, Antimonova and Komarova

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of KPILP expression stimulates TMV:GFP reproduction and intercellular spread. (A) GFP-expressing foci visualization under UV light in N. benthamiana leaves of control plants (on the right) and plants with up- (on the left, pPVX) and downregulated [in the middle, pPVX(frKPILP)] KPILP expression 4 days after agroinfiltration with TMV:GFP. (B) Percentage of TMV:GFP-expressing foci of different size. (C) Mean GFP fluorescence intensity in analyzed foci. Relative amount of MP (D) and GFP (E) RNA in analyzed leaves quantified using qRT-PCR. Mean values and standard error are presented in histograms (B–E). The data was analyzed using ANOVA. Bars without same letters indicate significant differences according to Tukey HSD at p<0.05.