Figure EV2. Astrocyte‐specific deletion of CD38 decreases expression of GFAP, s100β and Cx43 in the developing mPFC.

• A
  Representative western blots of CD38, GFAP, Cx43, NDRG2, VGlut1, PSD95, MAG, and β‐actin in the mPFC of ctrl^P10 and CD38 AS‐cKO^P10 mice at P21 (two animals per genotype are shown).
• B–H
  Relative optical densities of CD38, GFAP, Cx43, NDRG2, VGlut1, PSD95, MAG, and β‐actin normalized to the loading control β‐actin (n = 4 animals per genotype, two‐tailed unpaired Student's t‐test).
• I
  Representative western blots of CD38 and β‐actin in the mPFC of ctrl^P42 and CD38 AS‐cKO^P42 mice at P84 (two animals per genotype are shown).
• J
  Bar graphs depict the relative optical density of CD38 and β‐actin normalized to the loading control β‐actin (n = 3 animals per genotype, two‐tailed unpaired Student's t‐test).
• K–T
  (K, M, O, Q, S) Immunohistochemistry for GFAP, s100β, NDRG2 and SOX9 in the mPFC and MBP in the motor cortex of ctrl^P10 and CD38 AS‐cKO^P10 mice at P21. Nuclei were counterstained with DAPI. Scale bars, 50 μm (K, M, O, and Q), and 100 μm (S). (L, N, P, R, T) Quantification of astrocyte‐specific protein‐positive cells and MBP intensity (n = 4 animals per genotype, two‐tailed unpaired Student's t‐test).

Data information: Data represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

Source data are available online for this figure.