TABLE 2.
Physical characteristics of the viruses lacking E1 (Ad-LacZ) or E1 and fiber produced on 293 and 293-Fb cellsa
| Cells | Virus | Particles/ml | IU/ml | IU/particle |
|---|---|---|---|---|
| 293 | Ad-LacZ | 1.1 × 1012 | 1 × 1011 | 1/10 |
| Ad-LacZ/Fb°max | 4.4 × 1011 | 4.3 × 106 | 1/105 | |
| Ad-LacZ/Fb°min | 8.8 × 1011 | 6.65 × 106 | 1/105 | |
| 293-Fb | Ad-LacZ/Fb°max | 5.4 × 1011 | 4.1 × 108 | 1/103 |
| Ad-LacZ/Fb°min | 8.8 × 1011 | 7.4 × 108 | 1/103 |
a
Human 293 or 293-Fb cells (3 × 108) were infected with the indicated viruses at 3 IU/cell. At 40 to 72 h postinfection, the cells were recovered and the viruses were extracted and purified. The results shown are representative of three independent productions.