TABLE 2.

Spontaneous reactivation of LAT2.9A^a

Expt	Virusb	No. positive/total eye cultures (%)c	P vs LAT2.9Ad	P (fraction of positive cultures/eye vs LAT2.9A)e	No. positive/total eyes (%)	P vs LAT2.9Ad
1	LAT2.9A	11/156 (7.1)			5/6 (83.3)
	dLAT2903 (LAT−)	13/364 (3.6)			2/14 (14.3)
	LAT3.3A (wt)	38/312 (12.2)			10/12 (83.3)
	dLAT2903R (wt)	50/364 (13.7)			13/14 (92.9)
2	LAT2.9A	6/364 (1.6)			4/14 (28.6)
	dLAT2903R (wt)	52/624 (8.3)	<0.0001	0.02	16/24 (66.7)	0.04
3	LAT2.9A	1/184 (0.5)f			1/8 (12.5)
	LAT2.9AR	36/364 (9.9)	<0.0001	0.01	10/14 (71)	0.02
	dLAT371 (wt)	36/300 (12)g	<0.0001	0.03	8/12 (67)	0.7
	dLAT371R (wt)	63/527 (11)	<0.0001	0.01	15/22 (68.2)	0.01
Totalsh	LAT2.9A	18/704 (2.6)			10/28 (36)
	dLAT2903	45/1768 (2.5)	0.98	0.32	12/68 (18)	0.32
	Wild-type viruses	275/2584 (10.6)	<0.0001	0.01	72/100 (72)	0.0007

^aAnalyses of the experiment shown in Fig. 4. 

^bViruses designated “(wt)” have previously been shown to have spontaneous reactivation rates indistinguishable from that of the parental McKrae wild-type virus. 

^cVirus-positive tear cultures per total number of cultures. The number of cultures is not always equal to the number of eyes times 26 days (the length of the study period) because of occasional deaths apparently unrelated to the latent infection. 

^dFisher exact test. P < 0.05 indicates statistical significance. 

^eFor each eye within each group the fraction of days on which positive cultures were obtained was calculated and the resulting fractions (one per eye) were then compared between groups by using the Mann-Whitney rank sum test. The groups were considered significantly different if P was <0.05. 

^fOne rabbit died prior to tear collection on day 15. 

^gOne rabbit died prior to tear collection on day 3. One rabbit died prior to tear collection on day 19. 

^hSee Table 1, footnote d.