Comparison of the left-sided ORFs of AAV. The left-sided ORFs of AAV were aligned with the MACAW program. Segment pair overlap with a minimum segment pair score of 60 was used as a search method to define amino acid (aa) blocks of local similarity. The aligned blocks were linked and identical amino acid residues were shaded. Shown are alignments of AAV2, AAV3A, AAV3B, AAV4, and AAV6 (A) and AAV2, AAV3A, AAV3B, AAV4, AAV6, and AAV5 (B).