REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Clone RL2	Biolegend	Cat#677902; RRID:AB_2565912
Clone CTD110.6	Biolegend	Cat#838004; RRID:AB_2629520
GFPT1	Abcam	Cat#ab125069; clone EPR4854; RRID:AB_10975709
OGT	Abcam	Cat#ab96718; RRID:AB_10680015
OGA	Merck	Cat#HPA036141: RRID:AB_10672079
α-tubulin	Cell Signaling Technology	Cat#3873; clone DM1A; RRID:AB_1904178
Anti-mouse IgG peroxidase conjugate	Cell Signaling Technology	Cat#7076; RRID:AB_330924
Anti-mouse IgM peroxidase conjugate	Jackson Immunoresearch	Cat#115-035-020; RRID:AB_2338502
Anti-rabbit IgG peroxidase conjugate	Cell Signaling Technology	Cat#7074; RRID:AB_2099233
Bacterial and virus strains
E. coli BL21	in-lab	N/A
E. coli DH5alpha	in-lab	N/A
Chemicals, peptides, and recombinant proteins
Human Recombinant OGT	R&D systems	Cat#8446-GT
Alkaline phosphatase	Thermo Scientific	Cat#EF0651
Antarctic phosphatase	New England BioLabs	Cat#M0289
Human casein kinase II	New England BioLabs	Cat#P6010
Benzonase	Sigma-Aldrich	Cat#E1014
cOmplete™, EDTA-free Protease Inhibitor Cocktail	Roche	Cat#04693132001
HisPur Cobalt resin	Thermo Scientific	Cat#89964
GlcNAc-acceptor peptide (KKKYPGGSTPVSSANMM)	Nordic BioSite	Custom synthesis
Nunc® MaxiSorp™ 384	Sigma-Aldrich	Cat#P6116
UDP-GlcNAc	Sigma-Aldrich	Cat#U4375
Amplex UltraRed	Invitrogen	Cat#A36006
Experimental models: Cell lines
AML12 mouse hepatocyte cell line	ATCC	Cat#CRL-2254
AML12 cells overexpression wild-type GFPT1	This paper	N/A
AML12 cells overexpressing E328K mutant GFPT1	This paper	N/A
TU8988T pancreatic adenocarcinoma cell line	Kim et al. 202138	N/A
GFPT1 knockout TU8988T cells	Kim et al. 202138	N/A
HCT 116 Homo human colorectal carcinoma	ATCC	Cat#CCL-247
NIH/3T3 mouse embryonic fibroblast	ATCC	Cat#CRL-1658
Hepa1-6 mouse hepatoma	ATCC	Cat#CRL-1830
293T human kidney epithelium	ATCC	Cat#CRL-3216
HeLa human adenocarcinoma	ATCC	Cat#CRM-CCL-2
Experimental models: Organisms/strains
Congenic C57BL/6JCrl mice	Harlan	RRID:IMSR_JAX:000664
Oligonucleotides
Human OGT PCR cloning MunI-OGT 5′-ATCAATTGACCA TGCTGTGTCCCACCCATGCAGA-3′	Metabion	N/A
Human OGT PCR cloning: OGT-STP-SalI 5′-ATATGTCGAC TTATTCAACAGGCTTAATCATGTGGT-3′	Metabion	N/A
Mouse Gfpt1 PCR cloning: EcoRI-Gfpt1 5′-ATGAATTCGTGAC CAACATCATGTGCGG-3′	Metabion	N/A
Mouse Gfpt1 PCR cloning: Gfpt1-STP-XhoI 5′-ATCTCGAGTTAC TCTACTGTTACAGATTTGGC-3′	Metabion	N/A
Gfpt1 PCR mutagenesis (removal of internal EcoRI site), forward primer 5′-CTCATTATTTTTATCAGAGCGCTGG-3′	Metabion	N/A
Gfpt1 PCR mutagenesis (removal of internal EcoRI site), reverse primer 5′-TTCATTGTTATTCATAATGGAATCATCA-3′	Metabion	N/A
Gfpt1 E328K PCR mutagenesis, forward primer 5′-TCTTCTGCATAAATGAACTAAAGTTG-3′	Metabion	N/A
Gfpt1 E328K PCR mutagenesis, reverse primer 5′-AAATTTTTGAGCAGCCAGAATCTG-3′	Metabion	N/A
Recombinant DNA
Wild-type and E328K mutant Mus musculus Gfpt1 cDNA PCR amplified and cloned into pBluescript and Sleeping Beauty donor plasmid	This paper	GenBank accession for Gfpt1 cDNA sequence NM_013528
Software and Algorithms
GraphPad Prism 9	GraphPad Software	www.graphpad.com