Figure 2.
Efficient CSF cell cryopreservation validated by 3′ single-cell transcriptomics
(A) Post-quality control (QC) CSF cell counts in the fresh and cryopreserved (Cryo) sample pairs (p = 0.24).
(B) Uniform manifold approximation and projection (UMAP) plot of 21 clusters/cell states color coded by their annotations.
(C) UMAP indicating good representation of fresh and Cryo cells in each cluster.
(D) Annotation of clusters using selected marker genes. Heatmap colors correspond to the proportion of cells in each cluster expressing cluster marker gene.
(E) Bar chart indicating similar cell-type proportions in thirteen fresh and Cryo sample pairs.
(F and G) Significant positive correlation (F) between the different cluster proportions from thirteen fresh and Cryo sample pairs (p = 0.93) and (G) between two fresh-fresh sample pairs (p = 0.98, 0.99). Each point represents a cluster color coded by its sample ID.