REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
NeuN	Abcam	Cat# ab190195; RRID:AB_2716282
PV	Abcam	Cat# ab11427; RRID:AB_298032
SST	Millipore Sigma	Cat# MAB354; RRID:AB_2255365
CR	Sigma-Aldrich	Cat# C7479, RRID:AB_259024
PSD-95	Antibodies Incorporated	Cat# 75-028; RRID:AB_2292909
GFP	Thermo Fisher Scientific	Cat# A-31852; RRID:AB_162553
Anti-rat Fab2 A488	Jackson ImmunoResearch	Cat# 712-546-150; RRID:AB_2340685
Anti-rabbit Fab2 A594	Jackson ImmunoResearch	Cat# 711-586-152; RRID:AB_2340622
Anti-mouse IgG2a A488	Thermo Fisher Scientific	Cat# A-21131; RRID:AB_2535771
Chemicals, peptides, and recombinant proteins
NaCl	Sigma-Aldrich	Cat# S3014
NaHCO3	Sigma-Aldrich	Cat# S6014
NaH2PO4	Sigma-Aldrich	Cat# S3139
KCl	Sigma-Aldrich	Cat# P3911
Glucose	Sigma-Aldrich	Cat# G7528
MgCl2	Sigma-Aldrich	Cat# 63069
CaCl2	Sigma-Aldrich	Cat# 21115
mannitol	Sigma-Aldrich	Cat# M4125
MgSO4	Sigma-Aldrich	Cat# 83266
PBS	Sigma-Aldrich	Cat# P3813
acrylamide	Sigma-Aldrich	Cat# A9099
bisacrylamide	Sigma-Aldrich	Cat# M7279
potassium ferrocyanide	Sigma-Aldrich	Cat# 60279
NaN3	Sigma-Aldrich	Cat# 71289
Tween 20	Sigma-Aldrich	Cat# P9416
sodium cacodylate	Electron Microscopy Sciences	Cat# 12310
32% paraformaldehyde	Electron Microscopy Sciences	Cat# 15714
25% glutaraldehyde	Electron Microscopy Sciences	Cat# 16220
OsO4	Electron Microscopy Sciences	Cat# 19191
thiocarbohydrazide	Electron Microscopy Sciences	Cat# 21900
uranyl acetate	Electron Microscopy Sciences	Cat# 22400
EMbed 812 (EPON)	Electron Microscopy Sciences	Cat# 14121
acetonitrile	Electron Microscopy Sciences	Cat# 10021
Hoechst 33342 solution	Thermo Fisher Scientific	Cat# 62249
Streptavidin A488	Thermo Fisher Scientific	Cat# S11223
biotinylated WFA	Adipogen	Cat# VC-B-1355-M002
normal goat serum	Gibco	Cat# CN5000
Glycine	Invitrogen	Cat# 15527013
1N HCl	Fisher Scientific	Cat# SA48-500
EasyIndex solution (RI = 1.52)	LifeCanvas	N/A
VA044 Thermal Polymerization Initiator	Wako Chemicals	N/A
Experimental models: Organisms/strains
C57BL/6J	Jackson Laboratory	strain# 000664
Thy1-YFP-16	Jackson Laboratory	strain# 003709
Thy1-YFP-H	Jackson Laboratory	strain # 003782
Long-Evans	Charles River Laboratories	strain# 006
Software and algorithms
VAST	Berger et al.33	https://lichtman.rc.fas.harvard.edu/vast/