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(A) Full-scan mass spectrum of the synthetic MTX-dR reduced conjugate showing the singly protonated [M + H]+ at m/z 563.3 and the doubly protonated [M + 2H]+2 ion at m/z 282.3. (B) The MS2 scan stage mass spectrum of m/z 282.3 and (C) MS3 spectrum of the m/z 476.2 product ion are reported. (D) Proposed fragmentation mechanisms of the major ions.
