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MDA-MB-231 cells cotreated with 1 mM NNM and 0.6 μM MTX for 24 h. Reduced MTX-dR levels formed over 18 h in the isolated nuclei treated with NaB(CN)H3. AP site levels and stability in the nuclei of untreated and NNM-treated cells were measured at the same time points as done for MTX-dR reduction. Adduct levels are reported as the mean ± SEM (n = 4 independent replicates, ***P < 0.005, ****P < 0.0001, NS, not significant).
