Figure 2.

Induction of γδ T lymphocytes by HFD

(A) IL-17 serum levels in C57BL/6 mice fed an ND (3.3% fat) or HFD (35.7% fat) after the 3 day challenge with IMQ (n = 60).

(B and C) IL-17 production from different organs (B) and contribution of organs to IL-17 production (C) in IL-17 reporter mice fed an ND or HFD 3 days after being challenged with IMQ (n = 5–9). IL-17 production is defined as FITC-mean fluorescence intensity (MFI) ×IL-17A⁺ cell number.

(D) Representative dot plot showing IL-17⁺ γδ⁺ T lymphocytes pregated on CD3⁺ T cells in the spleen of mice fed an ND or HFD and challenged with IMQ.

(E and F) Number (E) and level (F) of IL-17 production (MFI) of IL-17⁺ γδ and αβ T lymphocytes in the spleen (n = 7–9).

(G) Contribution of IL-17-expressing cells to IL-17 production in the spleen. Bars show the mean ± SD.

p values were calculated by Welch’s t test (A) or unpaired Student’s t tests (B, E, and F) and are shown above each group.