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. 2023 Jul 7;42(7):112713. doi: 10.1016/j.celrep.2023.112713

Figure 3.

Figure 3

HFD reduces mucin expression in the intestine

C57BL/6 mice were fed an ND (3.3% fat) or HFD (35.7% fat) for 8 weeks and ear challenged with IMQ or without IMQ.

(A) Alcian blue periodic acid-Schiff (AB-PAS) staining of the ileum. Purple layers on the edge of the villus represents the mucin layer (black arrowhead), and mucin-producing cells are depicted by the blue-stained cells.

(A and B) Representative pictures and thickness of PAS layers are shown (A: n = 5; B: n = 10).

(B) Representative pictures of Mucin 2 staining in the ileum. Red: mucin 2; blue: DAPI.

(C) Quantitative real-time PCR for mucin mRNA expression in the first half of ileum (n = 10). Bars in the graphs show the mean ± SD. p values were calculated by unpaired Student’s t test.