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. 2023 Jul 7;42(7):112713. doi: 10.1016/j.celrep.2023.112713

Figure 7.

Figure 7

Akk reduced the increased ear IL-17 levels from HFD

(A) Representative dot plot showing IL-17+ γδ T lymphocytes pregated on CD3+ T cells in the whole IMQ-treated ear of ND- and HFD-fed mice with or without live or heat-killed Akk gavage.

(B) Flow cytometric analysis of the IL-17+ γδ T lymphocytes in the IMQ-treated ear skin. Histogram of IL-17A-GFP population, collective MFI, and number of IL-17+ γδ T lymphocytes in the whole IMQ-treated ear skin (n = 4–6).

(C) Fold induction of Il17a mRNA in the ear skin, as analyzed by qRT-PCR (n = 5).

(D) ELISA quantification of IL-17A concentration (pg/mL) in serum mice from the different groups (n = 5).

(E) Immunofluorescence for IL-17A (red) and DNA (DAPI, blue) and quantification of IL-17 cells/field in the IMQ-treated ear with or without HFD/AKK (n = 4–5). Data are presented as the mean ± SD. p values were calculated by analysis of variance followed by Sidak’s post hoc tests for comparison of groups indicated.