REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IgD–FITC	BD Biosciences	Cat. No. 555778
CD3-BV711	BioLegend	Cat. No. 317328
CD14-BV711	BioLegend	Cat. No. 301838
CD19-PE-Cy7	BD Biosciences	Cat. No. 560911
CD38-V450	BD Biosciences	Cat. No. 561378
CD138-APC	Miltenyi Biotech	Cat. No. 130-117-395
CD27-APC-e780	eBiosciences	Cat. No. 5016160
LiveDead	Invitrogen	Cat. No. L34966
IgD-Brilliant Violet 480	BD Biosciences	Cat. No. 566138
CD3-BUV737	BD Biosciences	Cat. No. 612750
CD14-BUV737	BD Biosciences	Cat. No. 612763
CD19-Spark NIR 685	BioLegend	Cat. No. 302270
CD38-Brilliant Violet 785	BioLegend	Cat. No. 303530
CD138-APC-R700	BD Biosciences	Cat. No. 566050
CD27-Brilliant Violet 711	BioLegend	Cat. No. 356430
BCMA-Brilliant Violet 421	BioLegend	Cat. No. 357520
TACI-PE-Cy7	BioLegend	Cat. No. 311908
OX40-Brilliant Violet 510	BioLegend	Cat. No. 350026
GITR-Brilliant Violet 605	BD Biosciences	Cat. No. 747664
Zombie NIR Fixable Viability Kit	BioLegend	Cat. No. 423106
IgD-BB700	BD Biosciences	Cat. No. 566538
CD3-PE-Cy5	ThermoFisher	Cat. No. 2363822
CD14-PE-Cy5	ThermoFisher	Cat. No. 2319032
CD19-BUV395	BD Biosciences	Cat. No. 740287
CD38-FITC	Cytognos	Cat. No. CYT-38F2-A
CD138-PE-Cy7	BioLegend	Cat. No. 356514
CD27-BV605	BD Biosciences	Cat. No. 562655
Live/Dead	ThermoFisher	Cat. No. L34962
Biological Samples
Bone marrow aspirate samples from a total of 11 healthy donors and 1 patient with high donor-specific antibodies awaiting kidney transplant.	Emory University and the ITN protocol (ClinicalTrials.gov Identifier: NCT04827979).	This paper.
Critical Commercial Assays
EasySep cell isolation kit	StemCell	Custom-designed
Zombie NIR Fixable Viability Kit	BioLegend	Cat. No. 423106
Chromium Single Cell Human BCR Amplification kit	10X Genomics	Cat. No. PN-1000253
Chromium Next GEM Single Cell 5′ Reagent Kit, v1	10X Genomics	Cat. No. PN-1000165
Deposited Data
scRNA-seq, scVDJ-seq raw and processed data	This paper	GSE230705
Original code	This paper	https://doi.org/10.5281/zenodo.7903579
Software and Algorithms
Cellranger 3.0.1	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/3.0/
Seurat 3.2.2	Butler et al.87	http://satijalab.org/seurat
SCTransform	Stuart et al.88	https://github.com/satijalab/sctransform
Slingshot	Street et al89	https://bioconductor.org/packages/release/bioc/html/slingshot.html
MAST	Finak et al.90	https://github.com/RGLab/MAST
GSEA v4.0.3	Subramanian et al.91	https://www.gsea-msigdb.org/gsea/index.jsp
UMAP	Becht et al.92	https://github.com/lmcinnes/umap
Molecular Signatures Database	Liberzon et al.37	http://software.broadinstitute.org/gsea/msigdb/index.jsp
STRING protein-protein interaction	Szklarczyk et al.38	https://string-db.org/