Fig. 2.

of SARS-CoV-2 from nasopharyngeal swabs or saliva samples by RT-LAMP: RNA was purified from nasopharyngeal swabs (blue) or saliva (red) from both qPCR positive (n = 12) and negative (n = 16) SARS-CoV-2 samples. RT-LAMP assay was performed with primers set ORF1a-HMSe (A) and Gene E (B) at 65°C. Reactions were performed at least in triplicate in two independent experiments. Water was used as a negative control and 200 viral copies per reaction were used as a positive control (data not shown). In reactions where no amplification was recorded, the “Time to Threshold” is reported as Not Detected (ND). The black lines represent the Mean and standard deviation (*p = 0.001). (C) Distribution profile of purified RNA from nasopharyngeal swab samples (blue) and purified RNA from saliva (red) between actual versus predicted residual in the Q-Q plot.