Fig. 3.

Comparison of detection of SARS-CoV-2 in RNA purified from saliva samples vs raw saliva by RT-LAMP: SARS-CoV-2 was detected in RNA purified from saliva samples (pRNA; red) or raw saliva (r-saliva; green) from both qPCR positive or negative SARS-CoV-2 samples by RT-LAMP with the primer sets (A) ORF1a-HMSe, (B) Gene E or (C) Gene N-A. Water was used as a negative control and 200 viral copies per reaction were used as a positive control (data not shown). In reactions where no amplification was recorded, the “Time to Threshold” is reported as Not Detected (ND). The black lines represent the mean and standard deviation. Reactions were performed at least in triplicate in two independent experiments.