Figure 5.

Trophectoderm cells derived from iPSCs with deleted cis-regulatory region of NDUFA4 show decreased ZIKV infection

(A and B) Western blotting analysis (A) and quantification (B) of NDUFA4 protein expression in trophectoderm cells derived from WT_Δ or NDUFA4^Δ hiPSCs. β-Actin was used as a loading control.

(C and D) Representative confocal images (C) and the quantification (D) of ZIKV-E staining in KRT7⁺ trophectoderm cells derived from WT_Δ or NDUFA4^Δ hiPSCs at 72 hpi (ZIKV^PR, MOI = 1). Scale bar = 50 μm.

(E) qRT-PCR analysis of (+) or (−) ZIKV vRNA strands of trophectoderm cells derived from WT_Δ or NDUFA4^Δ hiPSCs at 72 hpi (ZIKV^PR, MOI = 1).

(F) Viral titers of ZIKV in the supernatant of trophectoderm cells derived from WT_Δ or NDUFA4^Δ hiPSCs at 72 hpi (ZIKV^PR, MOI = 1) quantified by plaque assay. Data are representative of at least three independent experiments. p values were calculated by two-way ANOVA analysis; ∗∗∗p < 0.001.

See also Figure S5.