Antibodies |
|
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] |
GeneTex |
Cat #GTX57154; RRID: AB_2887950
|
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] |
Millipore |
Cat #MAB10216-I; RRID: AB_827205
|
Sox2 Antibody |
Cell Signaling |
Cat #3579; RRID: AB_2195767
|
Anti-NDUFA4 antibody |
Abcam |
Cat #ab129752; RRID: AB_11155881
|
Anti-Keratin 7 (D1E4) XP® |
Cell Signaling |
Cat #4465; RRID: AB_11178382
|
Anti-STEM121 human antigen |
Takara |
Cat #Y40410; RRID: AB_2801314
|
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat #A-21202; RRID: AB_141607
|
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher Scientific |
Cat #A-21203; RRID: AB_141633
|
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 594 conjugate |
Thermo Fisher Scientific |
Cat #A-21207; RRID: AB_141637
|
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 647 conjugate |
Thermo Fisher Scientific |
Cat #A-31573; RRID: AB_2536183
|
Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor 647 |
Thermo Fisher Scientific |
Cat #A-31571; RRID: AB_162542
|
DAPI |
Santa Cruz |
Cat# D1306; RRID: AB_2629482
|
|
Bacterial and virus strains |
|
ZIKVU (West Africa strain, MR766) |
ZeptoMetrix |
# 0810521CF |
ZIKVPR (Puerto Rico strain, PRVABC59) |
ZeptoMetrix |
# 0810521CF |
|
Chemicals, peptides, and recombinant proteins |
|
Y-27632 |
MedchemExpress |
#HY-10583 |
Recombinant Human bFGF Protein |
Peprotech |
#100-18B-500UG |
Recombinant Human BMP-4 Protein |
R & D Systems |
#314-BP |
StemFlex |
Gibco Thermo Fisher |
#A3349401 |
Knockout Serum Replacement |
Gibco Thermo Fisher |
#10828028 |
DMEM (high glucose) |
Gibco Thermo Fisher |
#11-965-118 |
Penicillin-Streptomycin (5,000 U/mL) |
Gibco Thermo Fisher |
#15070063 |
GlutaMAX Supplement |
Thermo Fisher Scientific |
#35050079 |
MEM Non-Essential Amino Acids Solution (100X) |
Gibco Thermo Fisher |
#11140050 |
Accutase |
Stem Cell Technologies |
# 07920 |
ReleSR |
Stem Cell Technologies |
# 05872 |
Matrigel |
Corning |
#354234 |
|
Deposited data |
|
Original western blot images |
This paper (Elsevier’s Mendeley Data repository) |
https://doi.org/10.17632/9y6cbwtwbg.1 |
|
Experimental models: Cell lines |
|
293T |
ATCC |
#CRL-11268 |
Vero E6 |
ATCC |
#CRL-1586 |
irradiated CF1 Mouse Embryonic Fibroblasts |
Thermo Fisher Scientific |
#A34180 |
|
Experimental models: Organisms/strains |
|
SCID-beige mice |
The Jackson Laboratory |
NA |
|
Recombinant DNA |
|
pLV[Exp]-EGFP:T2A:Puro-EF1A > hNDUFA4 |
VectorBuilder |
VB900004-0599xvh |
pLV[shRNA]-EGFP:T2A:Puro-U6>hNDUFA4[shRNA#1] |
VectorBuilder |
VB900052-8762rzm |
pLV[shRNA]-EGFP:T2A:Puro-U6>hNDUFA4[shRNA#2] |
VectorBuilder |
VB900052-8767wwk |
|
Software and algorithms |
|
Adobe illustrator CC2017 |
Adobe |
https://www.adobe.com/product/photoshop.html |
Graphpad Prism 8 |
Graphpad software |
https://www.graphpad.com |