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. 2023 May 29;26(7):107001. doi: 10.1016/j.isci.2023.107001
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] GeneTex Cat #GTX57154; RRID: AB_2887950
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] Millipore Cat #MAB10216-I; RRID: AB_827205
Sox2 Antibody Cell Signaling Cat #3579; RRID: AB_2195767
Anti-NDUFA4 antibody Abcam Cat #ab129752; RRID: AB_11155881
Anti-Keratin 7 (D1E4) XP® Cell Signaling Cat #4465; RRID: AB_11178382
Anti-STEM121 human antigen Takara Cat #Y40410; RRID: AB_2801314
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat #A-21202; RRID: AB_141607
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 Thermo Fisher Scientific Cat #A-21203; RRID: AB_141633
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 594 conjugate Thermo Fisher Scientific Cat #A-21207; RRID: AB_141637
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 647 conjugate Thermo Fisher Scientific Cat #A-31573; RRID: AB_2536183
Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat #A-31571; RRID: AB_162542
DAPI Santa Cruz Cat# D1306; RRID: AB_2629482

Bacterial and virus strains

ZIKVU (West Africa strain, MR766) ZeptoMetrix # 0810521CF
ZIKVPR (Puerto Rico strain, PRVABC59) ZeptoMetrix # 0810521CF

Chemicals, peptides, and recombinant proteins

Y-27632 MedchemExpress #HY-10583
Recombinant Human bFGF Protein Peprotech #100-18B-500UG
Recombinant Human BMP-4 Protein R & D Systems #314-BP
StemFlex Gibco Thermo Fisher #A3349401
Knockout Serum Replacement Gibco Thermo Fisher #10828028
DMEM (high glucose) Gibco Thermo Fisher #11-965-118
Penicillin-Streptomycin (5,000 U/mL) Gibco Thermo Fisher #15070063
GlutaMAX Supplement Thermo Fisher Scientific #35050079
MEM Non-Essential Amino Acids Solution (100X) Gibco Thermo Fisher #11140050
Accutase Stem Cell Technologies # 07920
ReleSR Stem Cell Technologies # 05872
Matrigel Corning #354234

Deposited data

Original western blot images This paper (Elsevier’s Mendeley Data repository) https://doi.org/10.17632/9y6cbwtwbg.1

Experimental models: Cell lines

293T ATCC #CRL-11268
Vero E6 ATCC #CRL-1586
irradiated CF1 Mouse Embryonic Fibroblasts Thermo Fisher Scientific #A34180

Experimental models: Organisms/strains

SCID-beige mice The Jackson Laboratory NA

Recombinant DNA

pLV[Exp]-EGFP:T2A:Puro-EF1A > hNDUFA4 VectorBuilder VB900004-0599xvh
pLV[shRNA]-EGFP:T2A:Puro-U6>hNDUFA4[shRNA#1] VectorBuilder VB900052-8762rzm
pLV[shRNA]-EGFP:T2A:Puro-U6>hNDUFA4[shRNA#2] VectorBuilder VB900052-8767wwk

Software and algorithms

Adobe illustrator CC2017 Adobe https://www.adobe.com/product/photoshop.html
Graphpad Prism 8 Graphpad software https://www.graphpad.com