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. 2023 Aug 1;23:505. doi: 10.1186/s12879-023-08472-w

Table 2.

Frequencies of bacterial species found by culture, Molecular Culture and 16S sequencing

Bacterial groups Bacterial species Culture Molecular Culture Illumina 16S sequencing
Gram positive Staphylococcus aureus 4 4 4
Staphylococcus lugdunensis 2 2 2
Coagulase-negative staphylococcia 1 2 2
Beta-haemolytic streptococcib 2 4 3
Viridans group streptococci 2 4 3
Corynebacterium spp. 2 3 3
Enterococcus spp.c 2 2 2
Micrococcaceaed 1 0 1
Gram negative Enterobacteralese 5 5 6
Pseudomonas spp.f 2 3 1
Anaerobes 0 5 5
Otherg 1 3 1

For a complete overview of detected bacterial species we refer to [Suppl. Material]

aExcept Staphylococcus lugdunensis

bAll were Streptococcus dysgalactiae

cAll were Enterococcus faecalis except for one sample where MC could not distinguish between E. faecalis and E. casseliflavus.

dIn both culture and 16S sequencing Kocuria sp. was identified.

eIncluding: Serratia marscecens, Serratia entomophila/nematodiphila, Citrobacter spp., Klebsiella spp. and Proteus spp.

fAll were Pseudomonas aeruginosa except for one sample where Pseudomonas sp. could not be characterised to species level by 16S sequencing.

gMycobacterium sp., Lactobacillus salivarius, Firmicutes spp., Enhydrobacter spp., Haemophilus parainfluenzae