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. 2023 Aug 1;42:190. doi: 10.1186/s13046-023-02775-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TNFAIP2 interacting with KEAP1 results in NRF2 stabilization. (a) IP/MS assay of TNFAIP2-interacting proteins. (b) Co-IP assay of the TNFAIP2 and KEAP1 interaction in HNSCC cell lines. (c) Flow cytometry analyses of ROS in TNFAIP2-overexpressing HNSCC cell lines with or without KEAP1 knockdown. (d-e) Cisplatin IC50 evaluations in TNFAIP2-overexpressing FADU (d) and CAL33 (e) cells with or without KEAP1 knockdown. (f) Western blot analysis of NRF2 in TNFAIP2-overexpressing HNSCC cell lines with or without KEAP1 knockdown. (g) Co-IP assay of the amount of NRF2 immunoprecipitated by KEAP1 in TNFAIP2 knockdown HNSCC cell lines treated with MG132 (10 µM, 4 h). (h) Co-IP assay of the amount of NRF2 immunoprecipitated by KEAP1 in TNFAIP2-overexpressing HNSCC cell lines. Data are presented as the mean ± SEM. n.s., not significant; *** P < 0.001. IP/MS, immunoprecipitation coupled with mass spectrometry