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. 2023 Feb 16;14(8):591–602. doi: 10.1093/procel/pwad008

Figure 1. .

Figure 1. 

AX improves developmental potential and genomic stability of murine ESCs. (A) Schematic illustration of the experimental design. (B) Colony morphology for ESCs cultured in 2iL or 2iLA at passage 3 or passage 15. Scale, 25 µm. (C) Developmental potential assessed by tetraploid complementation. The numbers at each timepoint represent total No. pups/No. embryos transferred. (D and E) Analysis for normal karyotype (D) in 2iL and 2iLA at different passages (n = ~20 metaphases at each passage for 2iL or 2iLA). Representative severe chromosome loss (E) in 2iL at P43. Arrow: Chromosome loss. Note both sister chromosomes were lost for chromosome 2, 4, 10, 15, 16, 17, 19 without any trisomy in this spread. (F) FACS analysis of XGXT reporter female ESC cultured in 2iL or 2iLA. The double reporter positive cells indicate both X chromosomes are maintained. ***P < 0.001, two-tailed t-test. (G) Karyotyping of the XGXT ESCs cultured in 2iL and 2iLA at passage 15. (H) LC–MS analysis of nucleotide pools for ESCs cultured in 2iL and 2iLA (P3). (I) Prps2 expression levels by qRT-PCR assay or Western blot assay. **P < 0.01, ***P < 0.001, multiple unpaired T-test.