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. 2023 Jan 31;56(8):e13411. doi: 10.1111/cpr.13411

FIGURE 3.

FIGURE 3

Upregulation of AC018926.2 rescued the PA‐induced osteogenic damage to PDLSCs. The cells were incubated in osteogenic medium with PA, and AC018926.2 was overexpressed in PDLSCs with lentivirus. (A) Overexpression efficiency of AC018926.2 in PDLSCs measured by qRT‐PCR. (B) Representative images of ALP staining and quantification of ALP activity in PDLSCs transfected with LV‐NC and LV‐AC018926.2 after osteogenic induction for 14 days (scale bar: 500 μm). (C) Representative images of Alizarin red S staining and quantitative analysis of the calcium mineral deposits formed by PDLSCs transfected with LV‐NC and LV‐AC018926.2 after osteogenic induction for 21 days (scale bar: 500 μm). (D) The effect of AC018926.2 overexpression on the expression levels of the osteogenesis‐related genes COL1, RUNX2, ALP, BMP2 and OCN in PDLSCs following 14 days of osteogenic induction measured by qRT‐PCR. (E) The effect of AC018926.2 overexpression on the osteogenesis‐related proteins COL1, RUNX2, ALP and BMP2 in PDLSCs following 14 days of osteogenic induction determined by Western blot analysis. All experiments were performed with 3 biological replicates. Data are presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 represent significant differences between the indicated columns, while NS represents no significant difference.