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. 2023 Jan 31;56(8):e13411. doi: 10.1111/cpr.13411

FIGURE 4.

FIGURE 4

Downregulation of AC018926.2 inhibited PDLSC osteogenic differentiation under a normal condition. The cells were incubated in normal osteogenic medium, and AC018926.2 was knocked down with specific siRNA. (A) Knockdown efficiency of AC018926.2 in PDLSCs measured by qRT‐PCR. (B) Representative images of ALP staining and quantification of ALP activity in PDLSCs transfected with si‐NC and si‐AC018926.2 after osteogenic induction for 14 days (scale bar: 500 μm). (C) Representative images of Alizarin red S staining and quantitative analysis of the calcium mineral deposits formed by PDLSCs transfected with si‐NC and si‐AC018926.2 after osteogenic induction for 21 days (scale bar: 500 μm). (D) The effect of AC018926.2 knockdown on the expression levels of the osteogenesis‐related genes COL1, RUNX2, ALP, BMP2 and OCN in PDLSCs following 14 days of osteogenic induction measured by qRT‐PCR. (E) The effect of AC018926.2 knockdown on the osteogenesis‐related proteins COL1, RUNX2, ALP and BMP2 in PDLSCs following 14 days of osteogenic induction determined by Western blot analysis. All experiments were performed with 3 biological replicates. Data are presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 represent significant differences between the indicated columns, while NS represents no significant difference.