Fig. 4.

The GPR35‐mediated KA sensing is closely related to the recovery of DSS‐induced colitis. (A) Schematic representation of the experimental design used. (B, C) The body weight (B) and disease activity score (C) of each rat were monitored daily, CID represents the GPR35 specificity inhibitor CID2745687. (D) The body weight and disease activity score were analyzed on day 17. (E) Western blot and quantification analyses of the protein expression levels of ERK1/2 and p‐ERK1/2 in the rat colon tissues. (F) Intestinal permeability analysis. (G) The relative change of the intestinal permeability prior to (day 10) and following termination of DSS exposure (day 17). (H) H&E and AB staining analyses of the rat colon tissues, scale bars = 100 μm (as indicated). The data are presented as mean ± SD. Statistical analysis was performed using the Student's t test. n = 6, *P < 0.05, **P < 0.01 and ***P < 0.001.