TABLE 3.
Normalized levels of DNA methylation at individual 5′-CG-3′ dinucleotidesa
| 5′-CG-3′ position | DNA methylation level in cell line:
|
|||
|---|---|---|---|---|
| BHK21 | T637 | BHK21-λ7 | BHK21-λ10 | |
| 1 | 1.000 | 1.942 | 1.070 | 1.186 |
| 2 | 1.000 | 1.591 | 1.390 | 1.353 |
| 3 | 1.000 | 1.489 | 1.192 | 1.199 |
| 4 | 1.000 | 1.509 | 1.094 | 1.431 |
| 5 | 1.000 | 1.117 | 1.087 | 1.046 |
| 6b | 1.000 | 1.387 | 1.179 | 1.179 |
| 7 | 1.000 | 1.233 | 1.090 | 1.148 |
| 8 | 1.000 | 1.317 | 1.205 | 1.185 |
| 9c | 1.000 | 1.253 | 0.991 | 1.259 |
| 10 | 1.000 | 1.278 | 0.947 | 0.947 |
| 11c | 1.000 | 1.066 | 0.970 | 1.005 |
| 12b | 1.000 | 1.381 | 0.807 | 1.163 |
| 13 | 1.000 | 1.259 | 0.947 | 1.183 |
| 14 | 1.000 | 1.313 | 0.892 | 1.143 |
| 15 | 1.000 | 1.232 | 0.854 | 1.152 |
| 16 | 1.000 | 1.121 | 0.844 | 1.133 |
| 17 | 1.000 | 1.471 | 1.020 | 1.025 |
| 18 | 1.000 | 1.193 | 0.737 | 0.969 |
| 20 | 1.000 | 1.893 | 1.571 | 1.476 |
| 21 | 1.000 | 2.212 | 1.478 | 1.921 |
| 22 | 1.000 | 1.855 | 1.153 | 0.987 |
| 28 | 1.000 | 1.569 | 0.989 | 0.812 |
| 29 | 1.000 | 1.557 | 1.006 | 0.980 |
| 30 | 1.000 | 1.548 | 1.012 | 0.875 |
| 31 | 1.000 | 1.528 | 1.521 | 0.894 |
| 32 | 1.000 | 1.137 | 1.099 | 1.000 |
| 33b | 1.000 | 1.317 | 1.394 | 1.233 |
| 34 | 1.000 | 1.324 | 1.259 | 1.173 |
a
See Table 2, footnote a. The values in Table 2 were normalized relative to the values for the reference cell line BHK21, which were set to 1.000. Boldface indicates the most significant increases in methylation at 5′-CG-3′ sites in the two λ DNA-transgenic cell lines.
b
HhaI (5′-GCGC-3′) sites.
c
HpaII (5′-CCGG-3′) sites.