TABLE 4.

Normalized levels of DNA methylation at individual 5′-CG-3′ dinucleotides in the IAPI (p3–p4) segment of the BHK21 cell line (reference), in cell line T637, and in four different nontransgenic BHK21 subclonal cell lines^a

5′-CG-3′ position	DNA methylation levels in cell line:
	BHK21 (68)b	T637 (43)	BHK21-1 (50)	BHK21-2 (60)	BHK21-3 (54)	BHK21-4 (60)
1	1.000	1.445	0.850	0.602	1.062	0.837
2	1.000	1.255	0.931	0.840	1.148	1.091
3	1.000	1.154	0.874	0.831	0.822	0.822
4	1.000	1.276	1.050	0.841	0.855	0.855
5	1.000	1.050	1.014	1.002	1.027	1.027
6c	1.000	1.149	0.996	1.010	1.010	1.010
7	1.000	1.094	0.899	0.987	0.946	0.938
8	1.000	0.999	0.900	0.823	0.910	0.901
9d	1.000	1.062	0.894	0.847	0.892	1.013
10	1.000	1.086	0.894	0.996	1.107	0.880
11d	1.000	1.058	1.076	1.014	1.120	1.125
12c	1.000	0.977	0.843	0.628	0.870	0.915
13	1.000	1.146	1.106	1.018	1.123	1.039
14	1.000	1.231	1.061	0.963	0.994	0.930
15	1.000	1.048	0.917	0.998	0.940	0.962
16	1.000	1.083	0.785	0.787	0.920	0.983
17	1.000	1.279	0.777	0.569	0.678	0.976
18	1.000	1.728	1.395	0.966	1.111	1.277
20	1.000	1.123	0.788	0.915	0.946	0.899
21	1.000	1.534	0.810	0.704	1.230	1.112
22	1.000	1.182	0.912	0.523	1.043	0.818
28	1.000	1.085	0.986	0.775	0.890	0.901
29	1.000	0.842	0.738	0.611	0.861	0.927
30	1.000	1.247	1.107	1.089	1.063	1.116
31	1.000	1.337	0.906	0.825	1.047	0.986
32	1.000	1.050	0.909	1.006	0.948	0.945
33c	1.000	1.194	0.891	0.992	0.982	1.049
34	1.000	1.213	0.933	0.999	1.090	1.035

^aExperimental details and methods of calculation are described in the footnotes to Tables 2 and 3 and the legend to Fig. 7. However, the data here are derived from completely independent sequence determinations of cloned PCR products in the IAPI p3–p4 segment (Fig. 6). The subclonal cell line BHK21-4 had been mock transfected, with λ DNA omitted from the transfection mixture. 

^bThe number of clones individually sequenced are given in parentheses. 

^cHhaI (5′-GCGC-3′) sites. 

^dHpaII (5′-CCGG-3′) sites.