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Comparison of normal CSS, GT, in the intron 6 of FAT4 and its mutant, AT, by in silico splice prediction tools and two predicted cryptic donor sites
| Sequences exon/intron | Position in intron 6 | NNSPLICE | NetGene2 | SD-score | |
|---|---|---|---|---|---|
| Normal CSS | GATTCAG/GTAAGTCC | +1 | 1.00 | 1–0.83 | −2.074 |
| Mutant CSS | GATTCAG/ATAAGTCC | +1 | - | - | - |
| Predicted donor sites | CATGGTG/GTGCGTGC | +191 | 0.74 | 0–0.41 | −3.998 |
| GACATGG/GTGAGTGA | +683 | 0.99 | 1–0.00 | −2.277 |
CSS, canonical splice site.
