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. 2023 Jun 24;22(8):100608. doi: 10.1016/j.mcpro.2023.100608

Fig. 3.

Fig. 3

Detergent-insoluble and whole proteome profiling from human AD brain samples.A, sample preparation of detergent-insoluble and whole brain proteome from AD cases and controls. B, total homogenate and the detergent-insoluble pellet (∼1 μg per sample) were analyzed by SDS-PAGE with molecular weight (MW) markers (kilodalton) followed by silver staining. C, TMT-LC/LC–MS/MS experiments for profiling detergent-insoluble and whole proteomes, in which the overlapped proteins and peptides are 7357 and 82,745, respectively. D, principal component analysis (PCA) of the top 1% variable proteins in detergent-insoluble proteome. E, heatmap showing the sample clustering with the top 1% most variable proteins in detergent-insoluble proteome. The expression levels were scaled by z-score for each protein. AD, Alzheimer's disease.