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. 2023 Jul 28;18(1):2241008. doi: 10.1080/15592294.2023.2241008

Figure 5.

Figure 5.

Hdac inhibition enhances microglial phagocytosis in a Nitric Oxide dependent matter. (a) Experimental design for phagocytosis assay and NO Assay. Media was collected for NO measurement prior to bead addition for phagocytosis. pHrodo beads were added for 1 hour and the percent of cells engulfing pHrodo beads was quantified by flow cytometry (b) Schematic of how Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) acts as a Nos inhibitor of which inhibits NO production and the predicted impacts on phagocytosis. (c) Example phase contrast and fluorescent images depicting enhanced bead engulfment by RGFP966 treatment at baseline and in response to LPS. Engulfed beads are shown in red. (d) Hdac3 inhibition blunts NO production in response to LPS. (e) Hdac3 inhibition enhances the percent of cells engulfing beads both at baseline and in response to LPS. (f) L-NAME blunts NO release in response to LPS and removes the impact of Hdac3 inhibition (g) L-NAME eliminates the impact of Hdac3 inhibition on phagocytosis following LPS treatment, but not at baseline. Mean ± SEM. n = 5–6 from 3 independent cultures *p < 0.05, **p<.01, ***p < 0.001, ****p < 0.0001.