FIGURE 3.

Vascular network features of the cortical surface of male and female WT and 5xFAD mice. Vessel density (A,E), junction density (B,F), total vessel length (C,G), and average vessel length (D,H) are shown for male and female WT and 5xFAD mice at 4, 8, and 12 months. The age effect reported in Figure 2 is driven primarily by males as female vascular features were relatively stable. Representative images of vessel painted hemispheres (left) with AngioTool resultant image (right) in a 4-month-old 5xFAD male (I) and a 12-month-old 5xFAD male (J) illustrate the increasing junctions. δ reports a significant effect of age (two-way ANOVA, ^δδδp < 0.001, ^δδδδp < 0.0001); for multiple comparisons (Sidak’s test): *p < 0.05, **p < 0.01, ***p < 0.001.