Table 2.
SerratiaAgDsRed is transferred from mosquitoes to mice ears during blood feeding.
| Mosquito species | Infected mouse/ Total mice | ||
|---|---|---|---|
| PCRa | Cultureb | Microscopyc | |
| An. stephensi | 4/9 | 4/9 | 3/9 |
| An. gambiae | 6/9 | 6/9 | 4/9 |
SerratiaAgDsRed-infected mosquitoes were allowed to blood feed on CD1 mice. Mouse ears were and processed immediately after mosquito blood feeding.
a
Diagnostic PCR for Ds-Red was performed on DNA extracted from ears of infected mice.
b
The ears of infected mice were homogenized, and bacteria grown in culture medium;
c
The ears of infected mice were longitudinally dissected and analyzed by confocal microscopy for the presence of DsRed fluorescent Serratia. A total of 9 mice was used for both mosquito species in three independent experiments.