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Ab initio heterogeneous reconstruction of an experimental dataset of the pre-catalytic spliceosome (EMPIAR-10180 [22]). (Top left) Distribution of the two first principal components of and sampling points along the first component. (Top right) Structures generated by traversing along PC1. The outline indicates the edges of volume (5). (Bottom) Distribution of the viewing directions. The “reference” shows the viewing directions published in [22].
