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. 2023 Jul 13;12:RP86972. doi: 10.7554/eLife.86972

Figure 1. Slc6a17 expression in the mouse brain.

(A) A schematic diagram illustrating the strategy for the generation of Slc6a17-2A-CreERT2 mice. Crossing of Slc6a17-2A-CreERT2 mice with Ai14 (LSL-tdTomato) mice allowed specific labeling of Slc6a17-expressing neuron after tamoxifen injection. More details in Figure 1—figure supplement 1A. (B–I) Representative coronal sections of Slc6a17-2A-CreERT2::Ai14 mice. Numbers above images indicate the anteroposterior position of the section from Bregma in millimeters (mm), based on Paxinos and Franklin, 2019. Scale bars = 1 mm. (J) Slc6a17-positive neurons in the basolateral amygdaloid nucleus (BLA) and the central amygdaloid nucleus (CeA). Scale bars = 100 μm. (K) Hippocampal expression of Slc6a17. Slc6a17-positive neurons are densely distributed in the granule cell layer of the dentate gyrus (GrDG), and CA3, with little expression in the CA1 and the CA2. Scale bars = 200 μm. Abbreviations: 2Cb, lobule 2 of the cerebellar vermis; 3Cb, lobule 3 of the cerebellar vermis; 4/5Cb, lobule 4 and 5 of the cerebellar vermis; AON, accessory olfactory nucleus; AM, anteromedial thalamic nucleus; AV, anteroventral thalamic nucleus; BLA, basolateral amygdaloid nucleus; Ent, entorhinal cortex; CB, cerebellum; Cg1/2, cingulate ccortex; CIC, central nucleus of the inferior colliculus; Ctx, cortex; Hip, hippocampus; LO, lateral orbital cortex; LS, lateral septal nucleus; M1, primary motor cortex; M2, secondary motor cortex; OB, main olfactory bulb; Pir, piriform cortex; Pn, pontine nuclei; Rt, reticular thalamic nucleus; S1, primary somatosensory cortex; S2, secondary somatosensory cortex; SCN, suprachiasmatic nucleus; SFO, subfornical organ; SuG, superficial gray layer of superior colliculus; Sim, simple lobule; Th, thalamus; V1, primary visual cortex; V2, secondary visual cortex; VPM, ventral posteromedial nucleus.

Figure 1.

Figure 1—figure supplement 1. Generation of Slc6a17 knock-in mice.

Figure 1—figure supplement 1.

(A) A schematic diagram illustrating the knock-in strategy for generating Slc6a17-2A-CreERT2 KI mice. This CRISPR/Cas9-mediated strategy allowed CreERT2 to be co-expressed with SLC6A17 protein fused in frame with a T2A sequence. Crossing Slc6a17-2A-CreERT2 mice with Ai14 (LSL-tdTomato) mice specifically labeled Slc6a17-expressing cells after tamoxifen injection. (B, C) Representative sagittal sections of Slc6a17-2A-CreERT2::Ai14 mouse brains at postnatal day 56. Scale bars = 1 mm. (D) A schematic diagram illustrating the knock-in strategy for generating Slc6a17-HA-2A-iCre KI mice. Slc6a17 was fused in-frame with 3 repeats of the HA tag, T2A, and iCre.