Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | SLC6A17 | GenBank | NM_001010898.4 | |
| Gene (H. sapiens) | Syp | GenBank | NM_009305.2 | |
| Strain, strain background (C57BJ/N) | Ai14 | Dr. Hongkui Zeng lab | ||
| Strain, strain background (C57BJ/N) | Rosa26-LSL-Cas9 | Jackson Laboratory | 028551 | |
| Strain, strain background (C57BJ/N) | Slc6a17 -2A-CreERT2 | CasGene Biotech (Beijing, China) | ||
| Strain, strain background (C57BJ/N) | Slc6a17-KO | CasGene Biotech (Beijing, China) | ||
| Strain, strain background (C57BJ/N) | Slc6a17 -HA-2A-iCre | Biocytogen (Beijing, China) | ||
| Strain, strain background (C57BJ/N) | Slc6a17 P633R | Biocytogen (Beijing, China) | ||
| Recombinant DNA reagent | pAAV-hSyn-SLC6A17- APEX2-WPRE-pA (plasmids) |
This paper | Supplementary file 1 | |
| Recombinant DNA reagent | pAAV-hSyn-SLC6A17- HA-WPRE-pA (plasmids) |
This paper | ||
| Recombinant DNA reagent | pAAV-hSyn-SLC6A17G 162R-HA-WPRE-pA (plasmids) |
This paper | ||
| Recombinant DNA reagent | pAAV-hSyn-DIO-Syp-HA-WPRE- pA-U6-3-t::gRNA WPRE-pA (plasmids) |
This paper | ||
| Recombinant DNA reagent | NEBuilder HiFi DNA Assembly Cloning Kit | NEB | E5520S | |
| Antibody | Anti-Synaptophysin (mouse monoclonal) |
Synaptic System | 101011 | IP 1:200 |
| Antibody | Anti-Synaptophysin (rabbit polyclonal) |
Synaptic System | 101 002 | IB 1:2000 |
| Antibody | Anti-Synaptotagmin 1 (rabbit polyclonal) | Synaptic System | 105 008 | IB 1:2000 |
| Antibody | Anti-VGLUT 1 (rabbit polyclonal) | Synaptic System | 135 302 | IB 1:2000 |
| Antibody | Anti-Proton ATPase (rabbit polyclonal) | Synaptic System | 109 002 | IB 1:2000 |
| Antibody | Anti-Synaptobrevin 2 (rabbit polyclonal) | Synaptic System | 104 202 | IB 1:2000 |
| Antibody | Anti-SV2A (rabbit polyclonal) | Synaptic System | 119 002 | IB 1:5000 |
| Antibody | Anti-VGAT (rabbit polyclonal) | Synaptic System | 131 002 | IB 1:2000 |
| Antibody | Anti-VGLUT2 (rabbit polyclonal) | Synaptic System | 135 402 | IB 1:2000 |
| Antibody | Anti-SNAP23 (rabbit polyclonal) | Synaptic System | 111 202 | IB 1:2000 |
| Antibody | Anti-GluN1 (mouse polyclonal) | Synaptic System | 114 011 | IB 1:5000 |
| Antibody | Anti-ERC 1b/2 (rabbit polyclonal) |
Synaptic System | 143003 | IB 1:5000 |
| Antibody | Anti-Synaptophysin (mouse monoclonal) |
Abcam | ab52636 | IF 1:500 |
| Antibody | Alexa Fluor 488 Anti-Synaptophysin (mouse monoclonal) |
Abcam | ab196379 | IF 1:200 |
| Antibody | Anti-Cathepsin D (rabbit monoclonal) | Abcam | ab75852 | IB 1:2000 |
| Antibody | Anti-PSMC6 (mouse monoclonal) | Abcam | ab22639 | IB 1:5000 |
| Antibody | Anti-GLUT4 (rabbit monoclonal) | Abcam | ab33780 | IB 1:2000 |
| Antibody | Anti-Transferring receptor (rabbit monoclonal) | Abcam | ab84036 | IB 1:2000 |
| Antibody | Anti-M6PR (rabbit monoclonal) | Abcam | ab124767 | IB 1:2000 |
| Antibody | Anti-LAMP2 (rabbit monoclonal) | CST | #49067 | IB 1:2000 |
| Antibody | Anti-EEA1 (rabbit monoclonal) | CST | #3288 | IB 1:2000 |
| Antibody | Anti-ERp72 (rabbit monoclonal) | CST | #5033 | IB 1:2000 |
| Antibody | Anti-Goglin-97 (rabbit monoclonal) | CST | #12192S | IB 1:1000 |
| Antibody | Anti-GM130 (rabbit monoclonal) | CST | #12480 | IB 1:2000 |
| Antibody | Anti-VDAC (rabbit monoclonal) | CST | #4661S | IB 1:2000 |
| Antibody | Anti-Syntaxin 6 (rabbit monoclonal) | CST | #2869 | IB 1:1000 |
| Antibody | Anti-LC3B (rabbit monoclonal) | CST | #2775 | IB 1:2000 |
| Antibody | Anti-HA (rabbit monoclonal) | CST | #3724S | IB 1:5000 IF 1:200 |
| Antibody | Anti-PSD95 (mouse monoclonal) | NeuroMab | 75028 | IB 1:5000 |
| Antibody | Anti-FLAG M2 HRP (mouse monoclonal) | Sigma | A8592 | IB 1:5000 |
| Commercial assay or kit | TMT10plex Isobaric Label Reagent Set | Thermo Scientific | 90113 | |
| Commercial assay or kit | Pierce High pH Reversed- Phase Peptide Fractionation Kit |
Thermo Scientific | 84868 | |
| Commercial assay or kit | Pierce protein G magnetic beads | Thermo Scientific | 88848 | |
| Commercial assay or kit | Pierce anti-HA magnetic beads | Thermo Scientific | 88837 | |
| Sequence-based reagent | KO sgRNA1 (5’- CGATGCTCCAGGCCACAAGGAGG -3’) | Michlits et al., 2020 | Targeting exon 5 | |
| Sequence-based reagent | KO sgRNA2 (5’-GCCGTGGCAGCATTGGTGTGTGG -3’) | Michlits et al., 2020 | Targeting exon 3 | |
| Sequence-based reagent | KO sgRNA3 (5’- TGGGCCTGGGCAACATCTGGAGG -3’) | Michlits et al., 2020 | Targeting exon 2 | |
| Chemical compound, drug | 3,3-Diaminobenzidine | Sigma | D5905 | |
| Software, algorithm | Noldus Video Tracking Software | Noldus | Ethovision XT 15 | |
| Software, algorithm | Prism 8 | GraphPad Software | ||
| Other | SeQuant ZIC-HILIC column (150 mm × 2.1 mm, 3.5 μm) |
Merck Millipore | 150442 | HPLC column for chemical detection |
| Other | SeQuant ZIC-pHILIC column (150 mm × 2.1 mm, 5 μm) |
Merck Millipore | 150460 | HPLC column for chemical detection |